Isolation and Characterization of Adhesive Secretion fromCuvierian Tubules of Sea Cucumber Holothuria forskali (Echinodermata: Holothuroidea)
The sea cucumber Holothuria forsk°ali possesses a specialized systemcalled Cuvierian tubules. During mechanical stimulation white filaments (tubules) are expelled and become sticky upon contact with any object. We isolated a protein with adhesive properties from protein extracts of Cuvierian tubules from H. Forsk°ali. This protein was identified by antibodies against recombinant precollagen D which is located in the byssal threads of the mussel Mytilus galloprovincialis. To find out the optimal procedure for extraction and purification, the identified protein was isolated by several methods, including electroelution, binding to glass beads, immunoprecipitation, and gel filtration. Antibodies raised against the isolated protein were used for localization of the adhesive protein in Cuvierian tubules. Immunostaining and immunogold electron microscopical studies revealed the strongest immunoreactivity in themesothelium; this tissue layer is involved in adhesion. Adhesion of Cuvierian tubule extracts was measured on the surface of various materials. The extracted protein showed the strongest adhesion to Teflon surface. Increased adhesion was observed in the presence of potassium and EDTA, while cadmium caused a decrease in adhesion. Addition of antibodies and trypsin abolished the adhesive properties of the extract.
Malgorzata Baranowska Ute Schloβmacher DouglasMcKenzie Werner E. G.M(u)ller Heinz C. Schr(o)der
Institute for Physiological Chemistry, University Medical Center of the Johannes Gutenberg Universit Xanthella Ltd., Marine Resource Centre, Barcaldine, Argyll, Scotland PA37 1SG, UK
国际会议
青岛
英文
1-13
2010-10-09（万方平台首次上网日期，不代表论文的发表时间）