Polymorphism and Balancing Selection of MHC Class II DAB Gene in 7 Selective Flounder ( Paralichthys olivaceus) Families
In order to determine the genetic variation of the MHC class IIB exon2 allele in the o.spring, 700 fry from seven families of Japanese flounder challenged with V. Anguillarum were studied, and di.erent mortality rates were found in those families. Five to ten surviving and dead fry from each of the seven families were selected to study the MHC class II B exon2 gene with PCR and a direct sequencing method. One hundred and sixteen di.erent exon2 sequences were found and 116 di.erent alleles were identified, while a minimum of four loci were revealed in the MHC class II B exon2 gene. The ratio (dN/dS) of nonsynonymous substitution (dN) to synonymous substitutions (dS) in the peptide-binding region (PBR) of the MHC class IIB gene was 6.234, which indicated that balancing selection is acting on the MHC class IIB genes. The MHC IIB alleles were thus being passed on to their progeny. Some alleles were significantlymore frequent in surviving than dead individuals. All together our data suggested that the alleles Paol-DAB*4301, Paol-DAB*4601, Paol-DAB*4302, Paol-DAB*3803, and Paol-DAB*4101 were associated with resistance to V. Anguillarum in flounder.
Min Du Song-lin Chen You Liang LeiWang Feng-tao Gao Yang Liu Xiao-Lin Liao
Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow S Key Lab for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow S
国际会议
青岛
英文
1-10
2010-10-09（万方平台首次上网日期，不代表论文的发表时间）