Distribution and Abundance of Archaea in South China Sea Sponge Holoxea sp. and the Presence of Ammonia-Oxidizing Archaea in Sponge Cells
Compared with bacterial symbionts, little is known about archaea in sponges especially about their spatial distribution and abundance. Understanding the distribution and abundance of ammonia-oxidizing archaea will help greatly in elucidating the potential function of symbionts in nitrogen cycling in sponges. In this study, gene libraries of 16S rRNA gene and ammonia monooxygenase subunit A (amoA) genes and quantitative real-time PCR were used to study the spatial distribution and abundance of archaea in the South China Sea sponge Holoxea sp. As a result, Holoxea sp. Specific AOA, mainly group C1a (marine group I: Crenarchaeota) were identified. The presence of ammonia-oxidizing crenarchaea was observed for the first time within sponge cells. This study suggested a close relationship between sponge host and its archaeal symbionts as well as the archaeal potential contribution to sponge host in the ammonia-oxidizing process of nitrification.
Fang Liu Minqi Han Fengli Zhang Baohua Zhang Zhiyong Li
Marine Biotechnology Laboratory, State Key Laboratory of Microbial Metabolism and School of Life Sci Eastern Hepatobiliary Surgery Hospital, Second Military Medical University, Changhai Road 225, Shang
国际会议
青岛
英文
1-5
2010-10-09(万方平台首次上网日期,不代表论文的发表时间)