Immune Efficacy of a Genetically Engineered Vaccine against Lymphocystis Disease Virus: Analysis of Different Immunization Strategies
Here, we report the construction of a vaccine against lymphocystis disease virus (LCDV) using nucleic acid vaccination technology. A fragment of the major capsid protein encoding gene from an LCDV isolated from China (LCDV-cn) was cloned into an eukaryotic expression vector pEGFP-N2, yielding a recombinant plasmid pEGFP-N2-LCDV-cn0.6 kb. This plasmid was immediately expressed after liposomal transfer into the Japanese flounder embryo cell line. The recombinant plasmid was inoculated into Japanese flounder via two routes (intramuscular injection and hypodermic injection) at three doses (0.1, 5, and 15 μg), and then T-lymphopoiesis in di.erent tissues and antibodies raised against LCDV were evaluated. The results indicated that this recombinant plasmid induced unique humoral or cell-mediated immune responses depending on the inoculation route and conferred immune protection. Furthermore, the humoral immune responses and protective e.ects were significantly increased at higher vaccine doses via the two injection routes. Plasmid pEGFP-N2-LCDV0.6 kb is therefore a promising vaccine candidate against LCDV in Japanese flounder.
Fengrong Zheng Xiuqin Sun Xing’anWu Hongzhan Liu Jiye Li SuqiWu Jinxing Zhang
First Institute of Oceanography, State Oceanography Administration of China, 6 Xianxialing Road, Qin Department of Microbiology, Fourth Military Medical University, 17 Changlexi Road, Xian City, Shanx Marine College, Shandong University, Weihai 264209, China
国际会议
青岛
英文
1-8
2010-10-09（万方平台首次上网日期，不代表论文的发表时间）