Biosynthesis and Immobilization of Biofunctional Allophycocyanin

摘要：

The holo-allophycocyanin-α subunit, which has various reported pharmacological uses, was biosynthesized with both Strep- II-tag and His-tag at the N-terminal in Escherichia coli. The streptavidin-binding ability resulting from the Strep II-tag was confirmed byWestern blot. Additionally, themetal-chelating ability deriving from theHis-tag not only facilitated its purification by immobilizedmetal-ion a.nity chromatography but also promoted its immobilization on Zn (II)-decorated silica-coatedmagnetic nanoparticles. The holo-allophycocyanin-α subunit with streptavidin-binding ability was thereby immobilized on magnetic nanoparticles.Magnetic nanoparticles are promising as drug delivery vehicles for targeting and locating at tumors. Thus, based on genetic engineering and nanotechnology, we provide a potential strategy to facilitate the biomodification and targeted delivery of pharmacological proteins.

作者: Yingjie Chen Shaofang Liu Yulin Cui Peng Jiang Huaxin Chen Fuchao Li Song Qin

作者单位: Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences,

会议类型: 国际会议

会议名称: 第九届国际海洋生物技术大会

会议地点: 青岛

会议语种:英文

页码: 1-6

在线出版日期: 2010-10-09（万方平台首次上网日期，不代表论文的发表时间）