Effects of Phytohormone Conditions on Callus Formation and Floral Differentiation from Styles in Crocus Sativus L.
In order to apply plant tissue cultural techniques on producing officinal compositions of saffron, styles of Crocus sativus Linn, as explants, the effects of various differentiation on callus formation and floral differentiation from styles were studied on the MS basic media, which was supplemented with various hormones, such as 6-BA , NAA, 2,4-D and IBA at different ratios. The results showed that these styles first dedifferentiated to callus on the suitable medium in early period, which further differentiated into bud-like structures, petal-like structures and stigma-like structures. It was found that NAA is the most effective auxin, and the following is IBA and 2, 4-D in the culture of styles. The ratio of 6-BA/NAA plays an important role in floral types, high ratio of 6-BA/NAA is helpful for callus to differentiate the stigma-like structures, while low ratio stimulates the formation of petallike structures.
Crocus sativus L. style callus floral differentiation in vitro flowering
Wang Hai-Ling Liu Wen-Qian Su Mei Tan Jian-Zhong
Department of Horticulture, Soochow University Suzhou, 215123, China
国际会议
The 2nd Conference on Key Technology of Horticulture(第二届园艺关键技术学术会议 CKTH 2010)
北京
英文
1-4
2010-07-17（万方平台首次上网日期，不代表论文的发表时间）