Cloning and Sequence Analysis of the Polygalacturonase (PG) Gene in Pepper (Capsicum Annuum L.)

摘要：

Cv. P98 of Pepper (Capsicum annuum L.) was used in this study. A full-length PG cDNA was isolated by rapid amplification of cDNA ends (RACE) and PCR, which designated as CaPG (GenBank accession number: FJ596175). The full-length CaPG gene contained a complete open reading frame (ORF) of 1107 bp, which encoded a 369 amino acids polypeptide. Amino acid sequence homo logy analysis of CaPG showed an 84％ identity with PG-2a in tomato and 85％ identity with PG in papaya. The result of phylogenetic analysis showed that PG gene from pepper and from tomato and papaya clustered together, and then came those from Arabidopsis.

关键词： polygalacturonase cDNA cloning pepper

作者: Chen Rugang Gong Zhenhui Yang Ruiping Li Dawei

作者单位: Northwest A&F University

会议类型: 国际会议

会议名称: The 2nd Conference on Key Technology of Horticulture(第二届园艺关键技术学术会议 CKTH 2010)

会议地点: 北京

会议语种:英文

页码: 53-56

在线出版日期: 2010-07-17（万方平台首次上网日期，不代表论文的发表时间）