Cloning and Expression of WRKY Gene Segment in Celery

摘要：

The WRKY transcription factor is considered to be involved in biotic and abiotic stress responses widely in plant. In this study, we acquired a 682 bp WRKY segment and a 1005 bp β -actin segment, induced WRKY gene expression in celery seedlings under the following treatment conditions: salinity cold (4℃), (200 mM NaCI), and abscisic acid (ABA) (100 μM), and finally detected different expressions of WRKY gene by using RT-qPCR (real-time relative quantification PCR). The results of RT-qPCR analysis revealed that the relative expression of WRKY gene improved 256.93％, 207.19％, 1825.37％ after cold, salinity, and ABA treatment respectively, which demonstrated that WRKY gene plays a positive regulatory role under different conditions.

关键词： WRKY transcription factor gene cloning gene expression RT-qPCR (real-time relative quantification PCR)

作者: Qin Song Fukuan Zhao Qingpeng Sun Aizhen Yang

作者单位: Department of Biotechnology, Beijing University of Agriculture, Beijing, China

会议类型: 国际会议

会议名称: The 2nd Conference on Key Technology of Horticulture(第二届园艺关键技术学术会议 CKTH 2010)

会议地点: 北京

会议语种:英文

页码: 69-73

在线出版日期: 2010-07-17（万方平台首次上网日期，不代表论文的发表时间）