Extraction of Protein from Stipe of Coprinus Comutus

摘要：

After separating the cap and stipe of Coprinus comatus, extraction method of protein in stipe was studied. Lipid was removed from stipe powder by soaking in ethanol, then protein was extracted with alkaline solution. The optimum extraction conditions deduced from response surface experiment are the following: stipe powder was extracted with 27 volumes of NaOH (pH 13) at 30 V for 0.5 h. For 100 g of stipe powder, 20.12 g of crude protein can be obtained. Its protein content was 41.05％, and polysaccharide was 19.67％. Using this method, protein in stipe can be effectively extracted while polysaccharide was less extracted. After extracting the protein of mushroom, the residue can be used to prepare the polysaccharide.

关键词： Coprinus comatus protein extraction stipe

作者: Li Bo Lu Fei Nan Haijuan

作者单位: Henan Institute of Science and Technology

会议类型: 国际会议

会议名称: The 2nd Conference on Key Technology of Horticulture(第二届园艺关键技术学术会议 CKTH 2010)

会议地点: 北京

会议语种:英文

页码: 78-81

在线出版日期: 2010-07-17（万方平台首次上网日期，不代表论文的发表时间）