Purification and Characterization of Superoxide Dismutase from the Raspberries
The raspberry is a resource rich in SOD, which were purified by the steps of salting out , gel chromatogram, ion exchang and metal-chelate affinity chromatography, some properties of raspberry SOD were studied as well. The main results were as follows: the specific activity and the recovery rate of the three enzymes from Metals Chelating Affinity Chromatogram were superior to that from DEAE-52. And molecular weight of SOD I is 24000D; SOD His 22000D; SOD III is 18000D. All the three enzymes were not sensitive to KCN and H2O2 · And the ultraviolet absorption peaks value of them were all 280nm. The effect of temperature on SOD I and SOD Ⅱ were small, but that on SOD III was bigger. The relative activity of SOD were higher when pH value was in range between 7 and 9. All the work have been done to provide foundation for further research of SOD and its application in hehlthy food.
Raspberry Superoxide dismutase purification
Li Bin Li Yuansu Meng Xianjun Xuan Jinghong
College of Food Science .Shenyang Agricultural University,China
国际会议
The 2nd Conference on Key Technology of Horticulture(第二届园艺关键技术学术会议 CKTH 2010)
北京
英文
108-112
2010-07-17（万方平台首次上网日期，不代表论文的发表时间）