Tissue Culture and Rapid Propagation Research of a New Species Medicinal Plant Gynura Medica
In order to establish the method of rapid propagation of Gynura Medica, explants derived from leaf and axillary bud of Gynura Medica were cultured on ten induction media containing different concentration combination of 6-BA and NAA to induce microshoot. Leaf callus induction rates of medium 3 is the highest, compared with the other nine media, up to 90.0％, while leaf callus hardly differentiate shoots. On all ten induction media, axillary bud can be reduced to form shoots with more than 40 shoots per axillary bud. Shoots induced from axillary bud were regenerated, and transferred to 6 rooting media. The rooting medium SGI is proper to induce shoots rooting. After hardening, shoots were transplanted, and survival rate is up to 98％. Gynura Medica can be rapid propagated through inducing microshoot from axillary bud to obtain a number of young plants.
Gynura Medica tissue culture callus N6-Benzyladenine(6-BA) α-Naphthaleneacetic acid. NAA) kinetin (KT)
FAN San-wei DONG Li-hui LING Qing-zhi TU Xiao-wei
Zhejiang Pharmaceutical College Ningbo, 315100, China
国际会议
The 2nd Conference on Key Technology of Horticulture(第二届园艺关键技术学术会议 CKTH 2010)
北京
英文
151-153
2010-07-17（万方平台首次上网日期，不代表论文的发表时间）