Comparative Investigation on in Vitro Regeneration of Different Rhubarb Cultivars
Differences in regeneration ability of five rhubarb cultivars were investigated using shoot tips of in vitro seedling on MS medium supplemented with 1.0mg/L NAA and 1.0mg/L 6-BA. Two different patterns of shoot proliferation were noted after 24 days of culture, i.e. caespitose shoots proliferating from callus of explants and axillary shoot bourgeoning from explants. A significant difference in regenerative ability was observed, with cultivar RT showing the highest multiplication coefficient (3.71/explant) and R19 presenting the lowest multiplication coefficient (1.96/explant). The result suggested that subculture for multiplication can be conducted in every 24 days to establish a micro-propagation system.
culinary rhubarb tissue culture regenerative ability
Yabei Wu Zuguo Cai Youduo Zhang Yipeng Zhao
Department of Horticulture and Landscape Architecture,Henan Institute of Science and Technology, Xinxiang 453003, China
国际会议
The 2nd Conference on Key Technology of Horticulture(第二届园艺关键技术学术会议 CKTH 2010)
北京
英文
157-159
2010-07-17（万方平台首次上网日期，不代表论文的发表时间）