Genetic Variation in Early-ripening Sports of Benitaka Grape Based on Random Amplified Polymorphic DNA
Random amplified polymorphic DNA (RAPD)-PCR analysis was applied for rapid investigation of the genetic variation between Benitaka grape (Vitis vinifera L) and early-ripening sports of Benitaka grape with the genome extracted by a modified CTAB method. A total of 280 random olignucleotide primers were assayed to obtain the highly reproducible and suitable primers for use in RAPD-PCR. The results show that only two primers OP A18 and OPB17 provided consistent and well reproducible band patterns, the polymorphic DNA fragments OP A18-800, OP A 18-450 and OPB17-500 produced by the two primers only present in the early-ripening sports of Benitaka grape, suggesting the potential use of those polymorphic DNA fragments as early-ripening mutation markers. The preliminary findings in this paper provide a basis for further understanding the molecular genetic basis of earlyripening sports of Benitaka grape. Further studies are required to investigate the genetic variation and mechanism of early-ripening sports of Benitaka grape, which might have important implication for further conservation and improvement of these early-ripening sports.
early-ripening sports RAPD-PCR genetic variation Benitaka grape
Zhao Yueling Wang Xiaowei Cheng Guanzhao Peng Zhenghua
Bio-Department of WeiFang University,WeiFang, 261061, China Life Science of Shandong University,JiNan, 250100, China
国际会议
The 2nd Conference on Key Technology of Horticulture(第二届园艺关键技术学术会议 CKTH 2010)
北京
英文
230-232
2010-07-17（万方平台首次上网日期，不代表论文的发表时间）