Effect of 2,4-dichlorophenoxyacetic Acid on Tissue Culture of Tree Peony Mature Embryo
Using the mature embryo of the tree peony cultivar viz. Fengdan as the explants, the effects of 2,4dichlorophenoxyacetic acid (2,4-D) on the culture of the tree peony mature embryo were primarily studied. The suitable concentration of 2,4-D in the callus induction medium of the mature embryo was defined. The results showed that, the frequencies of callus induction of the mature embryo were higher and the quality of callus was better when the concentration of 2,4-D in the medium was 1-7 mg/l. The optimization concentration of 2,4-D in the callus induction medium was 3 mg/l. The highest frequency of callus induction was 48.57％. It indicated that too, there was inhibiting effect of 2,4-D on the germination of mature embryos and the morphogenesis. This study has provided a theoretical basis for improving the tissue culture efficiency, establishing the simple and efficient in vitro clonal propagation system, genetic transformation and molecular plant breeding in the tree peony.
tree peony mature embryo culture 2,4-dichlorophenoxyacetic acid
Bi Ruiming
Department of Life Science, Heze University,Heze, the Peoples Republic of China
国际会议
2009 Academic Conference on Horticulture Science and Technology(2009园艺科学与技术学术会议 CHST 2009)
北京
英文
19-22
2009-12-12（万方平台首次上网日期，不代表论文的发表时间）