PI/DAPI Fluorochrome Staining Technique for Identifying Citrus Chromosomes

摘要：

The mitotic metaphase chromosomes of several citrus were stained with propidium iodide (PI) and 4-6diamidino-2-phenylindole (DAPI). The stable chromosome patterns were observed with the suitable proportion of PI/DAPI volume. Based on the relativesized PI signals and the morphology of no-signal chromosomes, 18 chromosomes of citrus were classified into 7 groups. In addition, the relative-sized PI signals on the chromosomes were observed maintain stable in the cell cycle. It is considered that the chromosome combined with PI fluorochrome were highly stable construction.

关键词： PI/DAPI staining chromosome citrus karyotype

作者: YU Yang WANG Wei YANG Xiao-ling

作者单位: College of Life Science and Technology,Tongji University Shanghai, China College of Life Science and Technology, Tongji University Shanghai, China

会议类型: 国际会议

会议名称: 2009 Academic Conference on Horticulture Science and Technology(2009园艺科学与技术学术会议 CHST 2009)

会议地点: 北京

会议语种:英文

页码: 53-55

在线出版日期: 2009-12-12（万方平台首次上网日期，不代表论文的发表时间）