A Shoot Regeneration from Anther Culture of Ziziphus jujuba Mill. Cv.Zanhuangdazao Hort.
Zinphus jujuba Mill. Cv. Zanhuangdazao Hort. is a triploid cultivar in natural. There are two ploidy level in its pollens, haploid and diploid. To obtain the regenerated plantlets from pollens, we studied the effects of basal medium, type of carbon source, darkness and cytokinin in anther culture of Ziziphus jujuba Mill. Cv. Zanhuangdazao Hort.. The callus induction and shoot regeneration of anthers were obtained on the same medium, i.e. MS (Murashige and Skoog) (1962) medium supplemented with maltose 20g/L > 2,4-dichlorophenoxyacetic acid (2,4-D) 1.0 mg/L and silver nitrate ( AgNOj ) 1.0 mg/L in the dark for 30 days with the highest percentage of anther ex plants that produced calli (793％), overall efficiency of regeneration (72.6％). By flow cytometry analysis of ploidy level, we first obtained the diploid shoots from pollens of Ziziphus jujuba Mill. Cv. Zanhuangdazao Hort. successfully.
anther culture callus induction shoot regeneration Ziziphus jujuba Mill Cv. Zanhuangdazao Hort
Na Wang Ziyu Qin Ling Qin Zhihui Zhao Mengjun Liu
Research Center of Chinese Jujube,Agricultural University of Hebei, 071001, Baoding, Hebei, P. R. Ch Department of Life Science, Hebei Normal University of Science and Technology, 066004 Changli, Hebe Research Center of Chinese Jujube, Agricultural University of Hebei, 071001, Baoding, Hebei, P. R. C
国际会议
2009 Academic Conference on Horticulture Science and Technology(2009园艺科学与技术学术会议 CHST 2009)
北京
英文
93-96
2009-12-12（万方平台首次上网日期，不代表论文的发表时间）