Stability of Exogenous CpTI Gene in Transgenic Apple Plantlets RepeatedlySubcultured in Vitro

摘要：

There were four transgenic lines of apple (Malus domestica Borkh.) carrying exogenous cowpea trypsin inhibitor (CpTI) gene including Royal Gala, Orin, Jonagold and Fuji as the test material. The stability of exogenous gene in transgenic lines conserved for 6 — 8 years by subculture at normal temperature were studied by the techniques of PCR analysis and kanamycin resistance evaluation. The PCR analysis results showed that all transgenic lines could generate the expectable band. The leaves of two lines presented yellow when the plantlets cultured on the medium with 50 mg/1 kanamycin, which indicated the lack of the kanamycin resistance, and the others could grow normally. So, the exogenous CpTI gene in transgenic apple plantlets repeatedly subcultured in vitro is stable.

关键词： apple npt II gene CpTI gene subculture stability

作者: Ruijin Zhou Huiling Hu Xiaoxin Shi Guoqiang Du

作者单位: College of Landscape Architecture,Henan Institute of Science and Technology Xinxiang, China College of Horticulture, Agricultural University of Hebei Baoding, China (author for correspondence)

会议类型: 国际会议

会议名称: 2009 Academic Conference on Horticulture Science and Technology(2009园艺科学与技术学术会议 CHST 2009)

会议地点: 北京

会议语种:英文

页码: 107-109

在线出版日期: 2009-12-12（万方平台首次上网日期，不代表论文的发表时间）