Establishment and Optimization of SRAP Reaction System in Litchi

摘要：

The impact factors of SRAP-PCR reaction, such as the template DNA amount, Mg2+ concentration, dNTP concentration, primer concentration, Taq DNA polymerase dosage were optimized by step optimization method. The results shew that 25 nL of the reaction system contained Mg2+ 2.0 mmolL, dNTPs 0.2 mmolL1, primer 0.3μmol·L-1, template DNA IS ng, and Taq DNA polymerase 1.50 U. The system was preliminarily carried out in a Fl population with 60 plants derived from MaguilixJiaohesanyuehong, and a clear, reproducible SRAP fingerprinting was obtained, which could be used for subsequent construction of genetic maps and other related research.

关键词： Litchi SRAP system optimization

作者: ZHAO Yu-Hui GUO Yin-Shan LI Kun LIU Cheng-Ming

作者单位: College of Horticulture, South China Agricultural University Guangzhou, 510642, China College of Hor College of Horticulture, Shenyang Agricultural University Shenyang, 110161, China College of Horticulture, South China Agricultural University Guangzhou, 510642, China

会议类型: 国际会议

会议名称: 2009 Academic Conference on Horticulture Science and Technology(2009园艺科学与技术学术会议 CHST 2009)

会议地点: 北京

会议语种:英文

页码: 114-117

在线出版日期: 2009-12-12（万方平台首次上网日期，不代表论文的发表时间）