Molecular Characterization and Expression of a cDNA Encoding a Single Myb-repeat Protein in Chimonanthus praecox
Chimonanthus praecox is an ornamental plant with great cold and drought tolerances. Based on the Chimonanthus praecox flower cDNA library construction and ESTs analysis, we obtained a CpMYBl protein gene, designated as CpMYBl, by sequencing randomly selected clones. The cDNA of CpMYBl is full length, and genomic DNA has no intron. CpMYBl contains a single conserved domain -SANT DOMAIN- in N terminal which shows that CpMYBl is a novel transcription factor belonging to MYB family from Chimonanthus praecox. The expression pattern of CpMYBl was analyzed by reverse transcription (RT)-PCR under some abiotic stresses, such as high temperature, low temperature, high salinity, drought and abscisic acid. The results showed that CpMYBl gene is expressed decreasingly, which proposed that CpMYBl is negatively correlated with abiotic stresses.
CpMYBl SANT/MYB domain abiotic stresses expression analysis Chimonanthus praecox
Sui Shun-zhao Luo Li-li Zhu Qing-long Pi Wei Luo Jiang-hui Ling Ming-yang
College of Horticulture and Landscape, Southwest University, Chongqing 400716, China Chongqing Engin College of Horticulture and Landscape, Southwest University, Chongqing 400716, China Key Laboratory College of Horticulture and Land cape, Southwest University, Chongqing 400716, China Chongqing Engin
国际会议
2009 Academic Conference on Horticulture Science and Technology(2009园艺科学与技术学术会议 CHST 2009)
北京
英文
180-183
2009-12-12（万方平台首次上网日期，不代表论文的发表时间）