Analysis of Subunit Composition of Hypocotyl Storage Proteins in Soybean Seed
Soybean seeds consist of two main parts, hypocotyls and cotyledons. The complexity of the subunits of hypocotyl storage proteins have not yet been well documented, although there are many reports on the synthesis of seed storage proteins in cotyledons. So, we investigated the complexity of hypocotyl storage proteins by two-dimensional electrophoresis and mass spectrometry, and found that the quantitative range among three types of seed storage proteins was smaller in hypocotyls than in cotyledons in dry mature seeds. In hypocotyls, only some subunits of the many subunits that are present in cotyledons were detected. This suggests the presence of functional differences among the hypocotyl storage proteins and their subunits.
soybean hypocotyls seed storage proteins Two-dimensional electrophoresis mass spectrometry
Tan Jian-Zhong Sun Bing-Yao Zheng Bi-Ping Fang Xiang-Ming Hirano Hisashi
Department of Horticulture, Soochow University, Suzhou 215123, China Department of Biology, Soochow University, Suzhou 215123, China Yokohama City University, Yokohama 230-0045, Japan
国际会议
2009 Conference on the Key Technology of Horticultural Produce(2009园艺生产关键技术学术会议 KTHP 2009)
北京
英文
48-51
2009-04-11（万方平台首次上网日期，不代表论文的发表时间）