A versatile dual-signal biosensor for analysis of samples without pretreatment
1. Introduction Detection of analytes in complex biological samples, such as pollutant and milk, normally requires sample pretreatment. These pretreatment regimes reduce assay throughput and increase testing costs. Technologies that make it possible to eliminate sample pretreatment are of great industrial interest. This is due to the fact that direct determination of biological samples would accelerate the assay development process as well as simplifying test routines As a consequence, there are considerable practical and economic incentives to develop testing regiments that eliminate the need for sample pre-treatment. Here we report the development of a dual-signal flow injected analysis device which eliminates the need for sample pretreatment. The device employs thermal traducers to measure the signal from an enzyme and a reference column. This makes it possible to independently monitor and correct for non-specifically generated heat, thereby eliminating the need for sample pretreatment. The ability of he dual-signal device to determine urea and lactate in milk samples without any prior treatment was evaluated.
Bin Xie
Department of Pure and Applied Biochemistry, Lund University, Sweden
国际会议
北京
英文
197-198
2011-11-24（万方平台首次上网日期，不代表论文的发表时间）