Development of Reverse Transcription Quantitative PCR assay for Detection and Quantification of Viable Pathogenic Bacteria in waters
1. Introduction Water quality standards related to sanitary security are usually based on measuring the concentrations of faecal indicator organisms (such as Escherichia coli and Enterococcus spp.) by culture-based methods. Since the RNA of bacteria has a short half-life in aquatic environment, it is a better marker for viable bacteria than DNA. The quantification of RNA is still questionable because of the expression of RNA is dependent on the physiological status of bacteria etc. The objective of this study was to establish a RT-Q-PCR method that could quantify viable bacteria based on RNA. Enterococcus faecium and Escherichia coli were studied and the genes coding for uidA and the 23S rRNA were selected as targets, respectively. In order to validate the established method, the real water samples that were inoculated with targets bacteria were studied.
Yi-wen Lin Dan Li Shu-xu Wu Miao He
State Key Joint of Environmental Simulation and Pollution Control (ESPC), Department of Environmental Science and Engineering, Tsinghua University, Beijing, 100084
国际会议
北京
英文
203-204
2011-11-24（万方平台首次上网日期，不代表论文的发表时间）