Novel Nano-immunosensor for 2,4-D Detection Based on Quantum Dot Fluorescence Resonance Energy Transfer
Introduction Semiconductor quantum dots (QDs) have emerged as promising alternative bioanalytical tools because of their unique optical properties, such as high quantum yield, photostability, narrow emission spectrum, and broad absorption. The narrow, sizetuned, and sym-metric emission spectra of QDs has made them excellent donors for fluorescence resonance energy transfer (FRET) sensors, and greatly reduces the overlap between the emission spectra of donor and acceptor and circumvents the cross-talk in such FRET pairs. QD-FRET biosensors have been widely used in immunoassay, clinical/diagnostic assays, and biomolecular binding assay. However, to date, few researches focus on detection of the small molecular pollutants in evironmental samples based on the principle of QD-FRET. In this study, we develop a QDFRET based competitive immunoassay method for high sensitive and selective detection of 2,4-D. 2,4-D belongs to the top ten hit-list of pesticides used all over the world, as it may lead 0to the occurr ence of cancer in humans, endocrine-disrupting activities and degenerative changes in nerve system.
Chunmei GU Jianwu SHENG Baodong SONG Feng LONG Miao HE Hanchang SHI
School of Environment, Tsinghua University, Beijing, 100084, P. R. China
国际会议
北京
英文
219-220
2011-11-24（万方平台首次上网日期，不代表论文的发表时间）