Selectively Detection of Infectious Pathogenic Bacteria in Environmental Waters by Fluorescent staining combined with Flow Cy tome try
1. Introduction Waterborne pathogens are one of the most significant threats to human health worldwide. Plaque assay is used as the traditional method for bacteria detection. But the results of plaque assay are not reliable as many bacteria are difficult to culture or are unculturable. And the procedure is very time-consuming, which need more than 24 hours. Fluorescent techniques combined with flow cytometry (FCM) have been used for assessment of the viability of microorganisms from different environment samples. 5-cyano-2, 3-ditolyl tetrazolium chloride (FCM-CTC), a kind of tetrazolium redox dyes can specifically stain the respiratory active bacteria. Furthermore, the combination of FCM-CTC and specific probe can effectively detect specific viable bacteria. The objective of present study was to establish an integrated specific probe-CTC-FCM assay. The assay was optimized based on the CTC concentration, incubation time, the fixative use and the specific probe concentration. Also, the validation of the assay was demonstrated.
Tian Yang Dan Li Miao He
State Key Joint Laboratory of Environmental Simulation and Pollution Control (ESPC), School of Environment. Tsinghua University. Beijing. 100841
国际会议
北京
英文
225-226
2011-11-24（万方平台首次上网日期，不代表论文的发表时间）