Perylene Diimide-Based Organic Fluorescent Nanorods for Live Cell Imaging
Cell imaging with fluorescent, magnetic or bimodal nanoparticles is currently undergoing a rapid development. The goal of this study was to evaluate the feasibility and efficiency of cell imaging by using a novel kind of organic fluorescent nanorods without any other dye staining or antibody labeling. Perylene-3, 4, 9,10-tetracarboxdiimide (PDI) nanorods with uniform diameters (ca. SO nm) and in a length range of 100-600 nm were fabricated by a templateassisted self-assembly method. The PDI nanorods showed a strong fluorescent emission in the visible range and favorable dispersity in aqueous solution. Human hepatoma (HepG2) cells, Hela cells and human embryonic fibroblasts (HEFs) were chosen as models to investigate the cytotoxicity and the cell imaging effect. MTT assays demonstrated the cytotoxicity of PDI nanorods was concentration-dependent and the 72 h IC50 value were in the range of 42-55 μg/mL which is much higher than that of reported quantum dots (QDs). Particularly, con-focal laser scanning microscopy (CLSM) and transmission electron microscopy (TEM) observations indicated PDI nanorods were able to be internalized into the cells very readily and mostly located in the cytoplasm. Moreover, they were found exhibiting a stable, bright fluorescence as long as several hours within the cells. Besides, the uptake of PDI nanorods was found through an energy-dependent, endocytosis-mediated process.
Meng-Meng Song Hong Bi Liang Wang Le-Le Yu
College of Chemistry and Chemical Engineering, Anhui University, Hefei 230039, P. R. China College of Chemistry and Chemical Engineering,Anhui University, Hefei 230039, P. R. China, Departmen College of Chemistry and Chemical Engineering,Anhui University, Hefei 230039, P. R. China
国际会议
2011 International Symposium on Bioelectronics and Bioinformatics(第二届国际生物医学电子学与生物信息学学术会议 ISBB 2011)
苏州
英文
166-169
2011-11-03（万方平台首次上网日期，不代表论文的发表时间）