Expression Analysis of Mirna Isomirs from Deep Sequencing Data
(0) Recently, several researches have reported the novel phenomena of microRNA isoforms (isomiRs), that microRNAs (miRNAs) can exhibit heterogeneous ends in 5 and 3 end, both addition and subtractionof diverse oligonucleotide. With the benefit of highthroughput sequencing technology, the expression profiles of miRNAs were analyzed here in the samples of the placentas of pregnant women after their delivery. By analyzing the profiles of miRNAs in four samples, we found three notable results. (1) The phenomenas of isomiRs were ubiquitous in all four samples. (2) The heterogeneous ends in 3end were more significantly than in 5 end, but showed no novel difference between the addition and subtraction of nucleotide in the ends. (3) The isomiRs with one and two nucleic acids heterogeneous took up approximately the same percentage with the mature miRNA in miRBase, and those isomiRs with more than 2 nt differences only took small part of the total miRNAs in the profiles. These results indicated the isomiRs maybe not just the inexact Dicer processing, but were the product of pre-miRNAs with some unknown but special bio-rules, which could give some impressing suspects for researches to better understand this novel phenomena ofisomiRs.
Qi Yang Jiafeng Lu Shengqin Wang Qinyu Ge Zuhong Lu
State Key laboratory of bioelectronics, Southeast University, Nanjing, P. R. China 210096 Key laboratory of Child Development and Learning Science, Ministry of Education State, Nanjing, P. R
国际会议
2011 International Symposium on Bioelectronics and Bioinformatics(第二届国际生物医学电子学与生物信息学学术会议 ISBB 2011)
苏州
英文
220-223
2011-11-03（万方平台首次上网日期，不代表论文的发表时间）