A laser scanning confocal imaging-surface plasmon resonance system application in real time detection of antibody-antigen interaction
The laser scanning confocal microscope (LSCM) offers several advantages over conventional optical microscopy, but most LSCM work is qualitative analysis and it is very hard to achieve quantitative detection directly with the changing of the fluorescent intensity. A new real time sensor system for the antibody-antigen interaction detection was built integrating with a LSCM and a wavelength-dependent surface plasmon resonance (SPR) sensor. The system was applied to detect the bonding process of human IgG and fluorescent-labeled affinity purified antibody in real time. The fluorescence images changing is well with that of SPR wavelengths in real time, and the trend of the resonance wavelength shift with the concentrations of antibody is similar to that of the fluorescent intensity changing. The results show that SPR makes up the short of quantificational analysis with LSCM with the high spatial resolution. The sensor system shows the merits of the of the LSCM and SPR synergetic application, which are of great importance for practical application in biosensor and life science for interesting local interaction.
laser scanning confocal microscope surface plasmon resonance sensor bonding process local interaction .
H. Y. Zhang L. Q. Yang W. M. Liu
Key Laboratory of Photochemical Conversion and Optoelectronic materials, Technical Institute of Phys Key Laboratory of Photochemical Conversion and Optoelectronic materials, Technical Institute ofPhysi
国际会议
桂林
英文
1-6
2011-11-01（万方平台首次上网日期，不代表论文的发表时间）