DEVELOPMENT OF ANALYTICAL METHODS FOR CELL CULTURE AND METABOLISM BASED ON COMBINATION OF MICROFLUIDIC DEVICES AND MASS SPECTROMETRY

摘要：

In this work, a method for the characterization of drug metabolites was developed by integrating chipbased solid-phase extraction (SPE) with on-line electrospray ionization quadrupole time-of-fight mass spectrometer (ESI-Q-TOF-MS). By integrating several parallel channels, the desalting and concentration process could be simultaneously achieved. The total sample pretreatment time only needed about 15 min, and solvent consumption could be reduced to less than one hundred microliters. All this demonstrated that the developed microfluidic device could be a potential useful tool for cellular drug metabolism research.

关键词： Microfluidic device cell metabolism mass spectrometry.

作者: Lin Jinming Gao Dan Wei Huibin

作者单位: Department of Chemistry, Tsinghua University, Beijing 100084, China

会议类型: 国际会议

会议名称: The 7th International Forum on Post-Genome Technologies and China-Japan-Korea Summit on Natural Products(第七届国际后基因组技术论坛及中日韩天然产物论坛 IFPT)

会议地点: 重庆

会议语种:英文

页码: 41-42

在线出版日期: 2011-10-28（万方平台首次上网日期，不代表论文的发表时间）