NANOSTRUCTURED METAL BIOSENSING SYSTEMS BASED ON LSPR FOR LABEL-FREE AND MULTIPLEX BIOSENSORS AND SERS FOR SINGLE CELL ANALYSES
Nanostructured metals have been studied for the localized surface plasmon resonance (LSPR) and electrochemical biosensors. Photonic plasmon spectra are caused by the refractive index variations that result from the binding of molecules to the metal nanostructures. There are optically detectable parameters in biophotonics and biosensor devices. We have studied three types of nanostructures, goldcapped nanostructure connecting with the core of silica nanoparticle capped by deposited gold film, gold-deposited porous anodic alumina layer chip and gold nanoparticles onto silicon oxide / silicon interferrometric multilayer as our original works. The bio-sensing of these nanostructures have been examined by monitoring the biomolecular interactions in various flexible formats. Antibody-antigen and DNA hybridization reactions were performed to detect various biomarkers, with the detection limit of picogram levels. The multi array format was constructed by a core-shell structured nanoparticle layer, which provided 300 spots on the sensing surface. A microfluidic biochip based on PDMS was useful for real-time analysis, rapid detection. DNA amplification process (PCR) and monoclonal antibody production from hybridoma cell library can be monitored. Electrochemistry measurements connecting to core-shell structure nanoparticle were successfully exploited in a simultaneous detectable scheme. The binding of melittin to lipid membrane was measured using localized surface plasmon resonance, and the permeability of the lipid membrane was then assessed electrochemically as a function of melittin with the purpose of seeking a novel, sensitive detection system for peptide toxins. Surface Enhanced Raman Scattering (SERS) was also discussed with gold and silver nanoparticles interacting with biomolecules. Gold nanoparticles were successfully delivered into single cells. Spatiotemporal measurements of SERS fingerprints suggested the dynamic molecular interactions and transformations taking place at different locations with time in cardiomyocytes.
Eiichi Tamiya
Nano-bioengineering and Biosensor group Department of Applied Physics Osaka University
国际会议
重庆
英文
61-62
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）