CARBON NANOSTRUCTURES AS ADSORBENTS FOR THE ISOLATION OF PROTEINS FROM BIOLOGICAL SAMPLES

摘要：

Multi-walled carbon nanotubes (MWCNTs) were used as sorbent for the isolation of basic proteins from biological samples via solid-phase extraction. A MWCNTs micro-column incorporated into a flow system facilitates on-line selective sorption of basic proteins, i, e., hemoglobin and cytochrome c. The retained protein species were afterwards separated from each other by their sequential elution from the micro-column employing appropriate eluents. A 0. 025 mol/L phosphate buffer solution of pH 8. 0 well facilitates the collection of hemoglobin, while a 0. 5 mol/L NaCl solution ensures a quantitative recovery of the retained cytochrome c.

作者: Du Zhuo Chen Meiling Liu Jiawei Chen Mingli Chen Xuwei Wang Jianhua

作者单位: Research Center for Analytical Sciences, Box 332, Northeastern University, Shenyang 10819, China

会议类型: 国际会议

会议名称: The 7th International Forum on Post-Genome Technologies and China-Japan-Korea Summit on Natural Products(第七届国际后基因组技术论坛及中日韩天然产物论坛 IFPT)

会议地点: 重庆

会议语种:英文

页码: 65-66

在线出版日期: 2011-10-28（万方平台首次上网日期，不代表论文的发表时间）