COMPARISON AND VERIFICATION OF GENOMIC DNA EXTRACTION BASED ON MAGNETIC NANOPARTICLES FROM ESCHERICHIA COLI AND WHOLE BLOOD

摘要：

In many large genetic studies, the amount of available DNA can be one of the criteria for selecting samples for study. Compared to those genomic DNA methods using organic solvents or commercial kits, the method based on MNPs is timesaving and cost-effective without the laborious centrifugation or precipitation steps, and has the great potential for automated DNA extractioa After the preparation of carboxyl-modified superparamagnetic nanoparticles, the yield and purity of genomic DNA based on MNPs are very better. In this paper, we compared and verify of genomic DNA extraction from Escherichia coli and whole blood based on magnetic nanoparticles. These genomic DNA extracted by using the MNPs method can all be available for the next molecule steps.

关键词： Magnetic nanoparticles genomic DNA extraction verification PCR method

作者: Ma Chao Liu Jiefeng Wang Chun Fang Wang Li Chuanyan Xi Zhijiang He Nongyue

作者单位: School of Chemistry and Life Science, Guangdong University of Petrochemical Technology, Maoming, Gua School of Chemistry and Life Science, Guangdong University of Petrochemical Technology, Maoming, Gua State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering School of Public Health, Southeast University, Nanjing 210096, China

会议类型: 国际会议

会议名称: The 7th International Forum on Post-Genome Technologies and China-Japan-Korea Summit on Natural Products(第七届国际后基因组技术论坛及中日韩天然产物论坛 IFPT)

会议地点: 重庆

会议语种:英文

页码: 84-86

在线出版日期: 2011-10-28（万方平台首次上网日期，不代表论文的发表时间）