CONSTRUCTION OF PROTEOME ANALYSIS SYSTEM WITH ULTRA PERFORMANCE SEPARATION USING LONG MONOLITHIC COLUMN

摘要：

Demands on high performance chromatographic separations of bio-related substances are increasing in the fields of proteomics. Recently, the monolithic column as a new separation medium attracts attention because of its potential for high performance separation in liquid chromatography. Long monolithic capillary columns were prepared, and the efficiency was examined using liquid chromatography. The fact that very high efficiencies were obtained for the retained solutes suggests the practical utility of the long monolithic capillary columns. In addition, the system using monolithic column was applied to proteome analysis and showed an efficient performance.

关键词： proteome analysis monolithic column proteomics nano-flow LC/ MS

作者: Hironobu Morisaka Mitsuyoshi Ueda

作者单位: Laboratory of Biomacromolecular Chemistry, Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa, Sakyo-ku, Kyoto 606-8502, Japan

会议类型: 国际会议

会议名称: The 7th International Forum on Post-Genome Technologies and China-Japan-Korea Summit on Natural Products(第七届国际后基因组技术论坛及中日韩天然产物论坛 IFPT)

会议地点: 重庆

会议语种:英文

页码: 87-88

在线出版日期: 2011-10-28（万方平台首次上网日期，不代表论文的发表时间）