DETECTION OF HEPATITIS B VIRUS DNA BY ELECTROCHEMICAL BIOSENSOR
The detection of hepatitis B virus (HBV) DNA have been become an important tool for the diagnosis of HBV infectioa In this work, an electrochemical DNA biosensor was prepared by immobilizing sulfydrylmodified ssDNA probe with particular DNA sequence of HBV on the gold electrode surface using self-assembly technique. The response of the biosensor to HBV DNA sequence was examined by Linear Sweep Voltammetry (LSV) using Hoechst 33258 as an electroactive indicator. The result showed there was a linear relationship between the concentration of complementary DNA with HS-ssDNA probe and A/p(the current difference of the oxidizaton peak). Furthemore, we have demonstrated the successful application of this biosensor for detecting HBV DNA in serum samples. It indicated that electrochemical DNA biosensor has great potential application in the recognition and detection of specific DNA.
Detection electrochemical DNA biosensor hepatitis B virus self-assembly
Xi Zhijiang Ma Chao Wang Fang He Nongyue
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering,Southeast University, Nanjing 210096, China
国际会议
重庆
英文
91-92
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）