HIGHLY EFFICIENT PRE-CONCENTRATION OF DNA AT A NEW MICRONANO JUNCTION
A new nanofluidic junction comprised of a capillary fracture and Nafion was prepared, and efficient DNA preconcentration at a very sharp point inside the capillary was achieved based on concentration polarization (CP) effect. Up to 800 V bias could be applied without any damage to the interface, and above 103 fold electropreconcentration of SYBR Green I labeled dsDNA was shown at the fracture inside the capillary within 15 s. The capillary chip could be used as a reliable platform for micro-nanofluidic studies, and play an important role for high efficient injection preconcentration for chip electrophoresis analysis for low abundance biomolecules.
capillary fracture Nafion nanofluidic concentration polarization DNA
Zhang Dawei Zhang Huiqin Tian Li Wang Lin Wu Zhiyong
Research Center for Analytical Science, Northeastern University, Shenyang 110819, China
国际会议
重庆
英文
106-107
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）