MAGNETIC NANOPARTICLE-BASED CHEMILUMINESCENCE DETECTION OF SEQUENCE-SPECIFIC HBV-DNA
With easy magnetic separation and large surface properties, magnetic nanoparticles have been employed as special biomolecule immobilizing carriers. In this paper, we reported a method of synthesizing and modifying SiO2/Fe2O4 (MNPs), followed by chemiluminescence (CD hybridization detection. The proposed methods take advantage of the high sensitivity and long stable light signal of the alkaline phosphatase (ALP) catalyzes the substrate AMPPD. The resulting core-shell structure MNPs coated with silica were spherical particles which had a good dispersion and consistent size at about 500 nra Based on this kind of MNPs, a protocol of chemiluminescence detection of sequence-specific DNA related to hepatitis B virus (HBV) was established. The optimization of relevant variables affecting the experiment and the sensitivity of this approach were also investigated. The MNPs-based method with high sensitivity and simple operation is expected to be employed in the detection of HBV serum both qualitatively and quantitatively and will have applications in simultaneous detection of various DNA sequences.
Magnetic nanoparticles Chemiluminescence HBV-DNA Hybridization
Wang Fang Ma Chao Zeng Xin Li Chuanyan Xi Zhijiang He Nongyue
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Nanjin State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Nanjin School of Public Health, Southeast University, Nanjing 210096, China
国际会议
重庆
英文
109-110
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）