OPTIMIZATION OF NUCLEIC ACID QUANTITATIVE ANALYSIS BASED ON MAGNETIC NANOPARTICLES AND CHEMILUMINESCENCE DETECTION
With combination of the merits of magnetic separation and chemiluminescence (CD, a novel approach for nucleic acid quantitative analysis is introduced and optimized in present study. The 5 amino-modified probes were immobilized onto carboxylated magnetic nanoparticles (MNPs), and then hybridized with the products extended with biotin-dUTP. After bonding with streptavidin-modified alkaline phosphatase (STVAP) and magnetic separation, the CL signals were detected by adding the substrate alkaline phosphatase (AP). The condition of carboxyl modification was optimized, and the best concentration of succinic anhydride (SA) is 0. 001 M, The result showed that the CL value of extension products is significantly higher than blank control, which suggested that this new method could be potentially applied in nucleic acid quantitation analysis.
Magnetic nanoparticles Chemiluminescence Quantitation analysis
Liu Ming Zeng Xin Sun Huanxiang He Nongyue
State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
国际会议
重庆
英文
114-116
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）