SILVER NANOCLUSTERS SYNTHESIZED VIA AN APTAMER-MEDIATED APPROACH AS AN INTRACELLULAR PROTEIN SENSOR
Ag nanoclusters (AgNCs) have attracted considerable attention due to their excellent photoluminescence, low toxicity, good photostability, and biocompatibility, which make them wide applications in the fields of chemical sensing, bioimaging, and single molecule microscopy. Here, we presented a facile approach to a new water-soluble, intense fluorescent AgNCs with yellow-emission under quasiphysiological conditions (pH 6. 6), which are stabilized by a sequence of DNA chimera via a chemical reduction method, and the DNA chimera with nanocluster templating sequence and PrPc aptamer acts as both a ligand to stabilize the metal core and a specific protein binding ligand. The fluorescent AgNCs probes can be used not only for the detection of cellular prion protein (PrPc) but also for PrPc imaging.
Ag nanoclusters prion protein aptamer
Zhou Yawen Huang Chengzhi
Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and C Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and C
国际会议
重庆
英文
130-132
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）