ABNORMAL EXPRESSION OF VEGF AND ITS DYNAMIC QUANTITATIVE ANALYSIS AT THE DIFFERENT STAGES OF HEPATOCELLULAR CARCINOMA DEVELOPMENT
Hepatocellular carcinoma (HCC) is becoming one of the most common malignant tumors worldwide and is characterized by a high vascularity. VEGF is a endothelial cell—specific vasculogenic and angiogenic growth factor, but its expression and role in HCC have not been extensively explored. The aim of this study was to investigate the dynamic expression and alteration of VEGF during HCC development. Rat hepatoma models were induced with 2-FAA on male SD rats. Morphological changes were observed by pathological examinations (HE staining). The expression of VEGF in rat liver tissues was semiquantitatively analyzed by immunohistochemistry. The VEGF levels in rat liver tissues and sera were detected by ELISA and Western blotting. Histological examination confirmed the rat hepatocytes from granule-like degeneration to atypical hyperplasia to HCC, and progressing increasing of hepatic VEGF levels during HCC development. The immunohistochemistry evidences indicated the positive expression and hepatocytes distribution of VEGF in rat hepatoma . The levels of hepatic or serum VEGF in degeneration, pre-cancerous and HCC groups were significantly higher than those in normal control group (P<C0. 01). The positive relationship of VEGF levels was found between liver tissues and sera of experimental rats (P<0. 01). Hepatic VEGF may participate in angiogenesis of hepatocyte canceration and detection of VEGF expression during HCC development could be a useful marker for early diagnosis of HCC.
HCC VEGF Immunohistochemistry Westen boltting ELISA
Qiu Liwei Yao Dengfu Wu Wei Sai Wenli Yang Junling Zhang Haijian
Research Center of Clinical Molecular Biology, Affiliated Hospital of Nantong University, Nantong 226001, China
国际会议
重庆
英文
150-152
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）