ANALYSIS FOR VARIATIONS OF BACTERIAL COMMUNITIES IN SMALL INTESTINE OF BKSDB MICE
The huge microbial communities play an important role in human physiology. More and more data shows that the intestinal flora, especially small intestinal flora, is closely related to metabolic disease, such as obesity and diabetes. In this study, small intestine contents samples of ten BKS-DB mice and 10 normal control mice were collected, followed by bacterial communities analysis using PCR-DGGE and cloning sequencing technology. We found that there were significant difference in Shannon-Weaver diversity index and Margalef index but no significant difference in Pielou index between experimental group and control group. This revealed that the normal mice have larger small intestinal flora than type 2 diabetes mice, while the dominant species and relative abundance were similar . The result of sequencing illustrated that Lactobacillus was in small intestine of normal control mice, but BKS-DB mice. This suggested that Lactobacillus was closely related to type 2 diabetes.
Type 2 diabetes BKS-DB mice Small intestinal flora DGGE
Hu Fang Zhou Dongrui Du Caihe Wei Tingting Zhang Renmin Zhang Honglin Lu Zuhong
State Key Laboratory of Bioelectronics, School of Biomedical Engineering. Southeast University,Nanji Research Center for Learning Science, Southeast University, Nanjing 210096, China State Key Laboratory of Bioelectronics, School of Biomedical Engineering. Southeast University,Nanji
国际会议
重庆
英文
153-155
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）