APTAMER-BASED TURN-ON FLURESCENT ASSAY FOR CELLULAR PRION PROTEIN DETECTION

摘要：

We have developed a novel turn-on fluorescent assay for the detection of cellular prion protein (PrPc) on the basis of a nucleic acid aptamer and a 3, 4, 9, 10-perylenetetracarboxylic diimide derivative (DAPER). In this PrPc detection system, the strong fluorescence of DAPER is almost completely quenched by the anti-PrPc aptamer (turn-off) j however, in the presence of PrPc, it binds to the DNA aptamer specifically and induces the unrestricted DNA fold into heparin structure, so the DAPER molecules are released that resulting in fluorescence recovery (turn-on).

关键词： Aptamer cellular prion protein DAPER label-free turn-on strategy

作者: Zhan Lei Liang Lijiao Zhen shujun Li Chunmei Huang Chengzhi

作者单位: Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and C College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China

会议类型: 国际会议

会议名称: The 7th International Forum on Post-Genome Technologies and China-Japan-Korea Summit on Natural Products(第七届国际后基因组技术论坛及中日韩天然产物论坛 IFPT)

会议地点: 重庆

会议语种:英文

页码: 156-158

在线出版日期: 2011-10-28（万方平台首次上网日期，不代表论文的发表时间）