DISPLAY TWO KINDS OF AMYLOLYTIC ENZYMES ON THE CELL SURFACE OF CORYNEBACTERIUM GLUTAMICUM, AND EFFECTIVE PRODUCTION OF GLUTAMATE FROM STARCH
The Corynebacterium glutamicum cells could not utilize starch as the carbon source to grow and produce amino acid. We previously constructed an recombinant strain diaplaying cramylase to hydrolyze starch materials in the medium. However, oramylase (AmyA) could not thoroughly hydrolyze starch into glucose, which was probably caused by the inability of AmyA to cleave a-1, 6-glycosidic bonds. Therefore, we furthermore displayed glucoamylase on the C. glutamicum cell surface by using the Cterminally truncated NCgl1221 as the anchor protein in this study. SDS-PAGE and Western blotting analysis confirmed that the fusion protein NCgll221glucoamylase was successfully expressed on the cell surface. TLC analysis showed that starch in the medium was exhausted by displayed AmyA and glucoamylase after 36 h of fernmentatioa The strain displaying two kinds of amylolytic enzymes produced more glutamate than that of the strain displaying AmyA only. The glutamate concentration in the medium was still increased after 22 h in the strain constructed in this study, when compared with that in the previously constructed strain. This study resolved the problem of incomplete consumption of the cheap carbon source-starch material.
Corynebacterium glutamicum Starch degradation Glucoamylase Surface display
Yao Wenjuan Zhang Wei Xu Xiaole Wang Yuqin Deng Xiaozhao
Department of Pharmacology, Nantong University Medical College, Nantong 226001, China Huadong Research Institute for Medicine and Biotechniques, Nanjing 210002, China
国际会议
重庆
英文
168-170
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）