EFFECT OF ANTI-TNF-o MAB ON NF-κB NUCLEAR TRANSLOCATION AND PROLIFERATION OF HUMAN HEPG2 CELLS
The activation of NF-κB induced by tumor necrosis factor-α (TNF-α) is closely related to hepatocarcinogenesis and drug tolerance, but the influence of inhibiting NF-κB Activation by TNF-α mAb in hepatoma cells is still poorly understood up to now. In this paper, we investigated the effect of TNF-α mAb on NF-κB activation and nuclear translocation by many methods, such as ELISA, Western Blot, qPCR, immunohistochemistry. The results indicated that the expression level of NF-κB mRNA was significantly inhibited. Besides, NF-κB activation and nuclear translocation were significantly inhibited by TNF-α mAb in HepG2 cells. So it can be seen that intervention with TNF-α mAb is expected to become a new method of HCC targeted therapy.
TNF-α mAb NF-κB cell cycle apoptosis
Zhang Haijian Yao Dengfu Wu Wei Qiu Liwei Qin Chenglin Yang Junling
Research Center of Clinical Medicine, Affiliated Hospital of Nantbng University, Nantong 226001, China
国际会议
重庆
英文
171-173
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）