EVALUATION OF A CO-DELIVERY COMPLEX COMPRISING A P53-BASED ANTITUMOR MINIPROTEIN AND A GENE
A novel lipid-coated ternary complex containing a p53-based peptide and a gene was developed by the electronic interaction-induced self-assembly of the ingredients. A newly designed antitumor miniprotein Stoppin, with a cluster of positive charges, was used as the starting core, followed by absorption of negative-charged plasmids and encapsulation with cationic lipids, under an optimized +/～/+ charge ratio of 1 : 2 : 4. The in vitro gene transfer properties of the complex were evaluated in human embryonic kidney 293T cells and the tumor-killing efficiency was assessed in human lung cancer line A549. Obviously enhanced GFP expression and 4-fold increased luciferase activity were observed when using two different plasmids to construct the ternary complexes respectively. Compared with peptide alone, a much stronger activity to tumor cells was also shown by the complex. Both enhanced gene delivery and antitumor effects suggest it might be a promising strategy to construct such a ternary complex for p53based synergistic cancer therapy in the future.
p53 co-delivery synergistic cancer therapy
Chen Zhangbao Li Chong Zhang Xiaolin Li Deng Xia Peng
College of Pharmaceutical Sciences, Southwest University, Key Laboratory on Luminescence and Real-Time Analysis, Ministry of Education, Chongqing 400715, China
国际会议
重庆
英文
174-176
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）