GRAPHENE OXIDE/ APTAMER FLUORESCENCE SYSTEM FOR DETECTION OF PRION PROTEIN
This article reports a graphene oxide (GO)-based fluorescent TAMRA-aptamer that achieves the rapid, sensitive, and selective detection of prion protein (PrPc) in homogeneous solution by exploiting interactions between GO-based fluorescent TAMRAaptamer and PrPc. The binding characteristics of graphene oxide / aptamers were studied using prion protein as a target Because of the extraordinarily high quenching efficiency of GO and guanine bases, the fluorescent TAMRA-aptamer exhibits minimal background fluorescence, while strong emission is observed when it was combined with the specific targets, leading to a high signal-to-background ratio. Our method improve the cognition to PrPc and provid evidence for the clinical diagnosis and therapeutics of prion disease. It is also demonstrated that this GO-based sensing platform is suitable for the detection of a range of analytes. It was found that PrPc ranging from 25. 6 to 128 μg/mL could be detected following our present desiga Therefore, our strategy is extremely simple and cost-effective.
TAMRA-aptamer GO fluorescence
Zhuang Honglin Huang Chengzhi
Education Ministry Key Laboratory on Luminescence and Real-Time Analysis. College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, China College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, China
国际会议
重庆
英文
180-181
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）