HEPATITIS C VIRUS F PROTEIN UP-REGULATES BCL-2 AND BAX IN HUMAN HEPATOMA HEPG2 CELLS
Objective It has been recognized that HCV(Hepatitis C virus) core protein plays an important role in liver cancer genesis, and F protein is a newly identified protein encoded by a +1 ribosomal frameshift. We investigate the regulatory effect of Hepatitis C Virus F protein on Bcl-2 and Bax. Methods Full length F gene of HCV was amplified by PCR, using the plasmid containing HCV lb full length open reading frame as template. HCV F gene containing, plasmid pEGFP-C3-F and HCV core gene-containing pEGFP-C3-C were constructed and transiently transfected into HepG2 cells. 48h posttransfection, Western blotting was used to determine the changes at translation levels of Bcl-2 and Bax genes. Results the levels of Bcl-2 expression were a little higher in pEGFP-C3-F transfected HepG2 cells than that of non-transfected cells, but significantly higher levels of Bax expression. Conclusion F protein is of regulatory properties in cellular oncogen Bcl-2 and anti-oncogen Bax, which may be implicated in the formation of hepatocelluar carcinoma.
Hepatitis C virus F protein Bcl-2 Bax
Wei Juan Deng Xiaozhao Kong Jing Chu Chunli Zhang Jinhai Yue Ming Lu Weidong Yu Xiaojie
School of Basic Medicine, Nanjing Medical University, Nanjing 21002, China Huadong Research Institute for Medicine and Biotechniques, Nanjing 210002, China Huaiyin Institute of Technology, Huaian 223300, China China Pharmaceutical University Nanjing 10316, China Kunming Medical College, Kunming 650031, China
国际会议
重庆
英文
182-184
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）