PROTEOMICS ANALYSIS OF CLEAR CELL RENAL CELL CARCINOMA: IDENTIFICATION OF POTENTIAL TUMOR MARKERS
Objectives: Due to the currently inefficient therapies and the low 5-year survival rates of RCC patients, novel diagnostic, prognostic, and therapeutic markers are needed for this disease. Method: The cancer tissues and paracancer normal tissues were preserved in liquid nitrogea In this report we analyzed the renal cell carcinoma tissues and paracancer normal tissues by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). The 16 differentially expressed protein spots (p<0. 05 by Student t-test) were identified by MALDI-TOF-MS and MS/MS. Results: 6 proteins were downregulated and 10 proteins were upregulated in clear cell RCC. The downregulated proteins were calbindin, ester hydrolase cllorf54, alcohol dehydrogenase, ammecrllike protein, ADP/ATP translocase 3 and LYR motifcontaining protein 5. The upregulated proteins were HIG1 domain family member 1A, Glutathione Stransferase P, Thioredoxin-dependent peroxide reductase, peroxiredoxin-6, CD2-associated protein, Annexin A5, gamma-enolase, retinal dehydrogenase 1, vimentin, and protein-glutamine gammaglutamyltransferase 2. Conclusion: In brief, our data provide potential tumor markers for diagnosis of RCC.
Renal cell carcinoma Proteomics analysis tumor markers
Sun Chuanyang Li Jinmei Sun Wei Hou Chuansheng Zhang Liang
Urology department, the second affiliated hospital of Soochow University, Suzhou, China School of Pharmacy, Medical College of Soochow University, Soochow University, Suzhou, China
国际会议
重庆
英文
202-204
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）