PULMO-TRACKING OF GLUTATHIONE S-TRANSFERASE IN MAMMAL
Targeted delivery of macromolecules into the lung has not yet been achieved. Here we showed that glutathione S-transferase (GST) is able to selectively introduce the biologically active enzyme choline acetyltransferase (ChAT) into the mouse lung. GST and GST-fusion proteins were prepared and injected intravenously 200 fig per mouse, and the GST and ChAT activities in different tissues were determined by specteophotometry method. The results indicated that GST intravenously injected targets dominantly to the lung, and effectively introduced ChAT into the lung. These results will open a new possibility for the direct delivery of therapeutic proteins into patients for lung diseases.
Glutathione S-transferase lung selectivity fusion protein protein therapy
Fu Ailing Yan Haitao
School of Pharmaceutical Sciences, Southwest University, Chongqing 400715, China Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, Chin
国际会议
重庆
英文
208-210
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）