RNA POLYMERASE II AS A LINK BETWEEN ALTERNATIVE SPLICING AND CHROMATIN STRUCTURE
Alternative splicing is a crucial mechanism to generate complexity of the proteome, yet its regulation is an open puzzle. Recent evidence indicates that signature of histone modifications and nucleosome positioning may cause splice site switching, suggesting RNA polymerase IKRNAP II) may mediate the link. Here we present analyses of RNAP II islands and nucleosome enrichment level around skipped exon(SE) 3 splicing sites and constitutive exon(CE) 3 splicing sites(3 ss). We have found histone modification plays more important role in slowing down RNAP II around SE 3 ss in contrast to CE 3 ss. Then we examined whether histone marking around these sites were special, finding that H3K4me3, H4K20mel and H3K27ac marks were different around SE and CE 3 ss in CD4+ T celL.
splicing chromatin transcription
Yu Shuangxin Huang Huan Liu Hongde Sun Xiao
State Key Laboratory of Bioelectronics Southeast University, Nanjing, China
国际会议
重庆
英文
214-216
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）