THE EFFECT OF HCV F AND CORE PROTEIN ON THE APOPTOSIS OF HEPG2 CELLS
The F protein is expressed by a +1 ribosomal frameshift during HCV translation. Discovery of F protein expressed from the HCV core coding sequence challenges properties assigned to core protein. In this study, we aimed to investigate the functional role of F protein in the change of cell-cycle on cellular levels. Cells were stably transfected using Lipofectamine 2000 with a EGFP reporter construction of different plasmids, pEGFP-F or pEGFP-core. Fluorescence microscope and Western blotting assays were performed at 48h of post-transfection to confirm successful transfections. Cells transfected with pEGFP-F or pEGFP-core plasmids were selected and tested to show that differences in their growth rates are due to endogenous core or F expressioa We concluded that F protein inhibits apoptosis of transfected cells, contrast to parent cells, but less than core protein.
Hepatitis C virus(HCV) F protein core protein Apoptosis
Chu Chunk Deng Xiaozhao Kong Jing Wei Juan Zhang Jinhai Yue Ming Lu Weidong Yu Xiaojie
China Pharmaceutical University, Nanjing 210009, China Nantong Tichen Health School, Nantong 220007, Huadong Research Institute for Medicine and Biotechniques, Nanjing 210002, China Huaiyin Institute of Technology, Huaian 223300, China Nanjing Medical University Nanjing 210029, China China Pharmaceutical University, Nanjing 210009, China Kunming Medical College, Kunming 650031, China
国际会议
重庆
英文
223-225
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）