A REGULATION MECHANISM FOR CD274 OVER-EXPRESSION IN GASTRIC CANCER
CD274 expresses in many cancers including gastric cancer, and is closely associated with disease progression, but the regulation mechanism remains largely unknown. In this study, we first confirmed CD274 expression in 42. 9％ of gastric cancer tissues, but not in normal tissues, and CD274 mRNA expression in both cancer and normal tissues, suggesting that CD274 expression is post-transcriptionally regulated. Hereafter, we sequenced the CD274 3-UTR and found more than half of gastric cancers (62. 4％) underwent G-to-C somatic mutations, which were directly correlated to the CD274 over-expression (p=1. 44 × 1010). From the luciferase reporter assays, we discovered that the CD274 expression on gastric cancer cells was down-regulated by miR-570, which was changed by the somatic mutation in theCD274 3-UTR and therefore contributed to elevated CD274 expression. These data showed an alternative novel mechanism for regulating CD274 expression in gastric cancer.
CD274 miR-570 somatic mutation gastric cancer
Wang Weipeng Sun Jing Li Fang Gu Yongping Zhang Xueguang
Department of Pharmaceutical Analysis, College of Pharmaceutical Science Medical Biotechnology Institute Department of Pathology, Medical College, Soochow University, Suzhou, China
国际会议
重庆
英文
233-235
2011-10-28（万方平台首次上网日期，不代表论文的发表时间）